Journal
SCIENTIFIC REPORTS
Volume 8, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-018-26577-0
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Funding
- National Institute of Mental Health's Psychoactive Drug Screening Program [HHSN-271-2008-00025-C]
- ShanghaiTech University
- Natural Science Foundation of Shanghai [16ZR1448500]
- NIH Intramural Research Program (NIDDK) [ZIA DK031116]
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Many hemorheologic Traditional Chinese Medicines (TCMs) that are widely-used clinically lack molecular mechanisms of action. We hypothesized that some of the active components of hemorheologic TCMs may function through targeting prothrombotic P2Y(1) and/or P2Y(12) receptors. The interactions between 253 antithrombotic compounds from TCM and these two G protein-coupled P2Y receptors were evaluated using virtual screening. Eleven highly ranked hits were further tested in radioligand binding and functional assays. Among these compounds, salvianolic acid A and C antagonized the activity of both P2Y(1 )and P2Y(12) receptors in the low mu M range, while salvianolic acid B antagonized the P2Y(12) receptor. These three salvianolic acids are the major active components of the broadly-used hemorheologic TCM Danshen (Salvia militorrhiza), the antithrombotic molecular mechanisms of which were largely unknown. Thus, the combination of virtual screening and experimental validation identified potential mechanisms of action of multicomponent drugs that are already employed clinically.
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