Journal
RSC ADVANCES
Volume 8, Issue 33, Pages 18266-18271Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8ra00492g
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Funding
- Natural Science Foundation of China [21405067, 21527808, 21575054]
- Fundamental Research Funds for the Central Universities [lzujbky-2017-199]
- '13th 5 year Plan' Program of the Jilin Education Department [JJKH20170433KJ]
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Change in cation concentration, including that of potassium and sodium, is characteristic of apoptosis, therefore it is significant to detect cation concentration changes. In this work a rapid, sensitive, and practical method was developed for the determination of Na+ and K+ concentration in HeLa cells during emodin induced apoptosis by a low-cost capillary electrophoresis device with capacitively coupled contactless conductivity detection (CE-(CD)-D-4). Under the optimized conditions, both ions were baseline separated in 4 min with 40 mM MES/40 mM His containing 1 mM 18-crown-6 as the separation buffer at pH 6.0. The limit of detections (LODs) and limit of quantifications (LOQs) were 0.47-1.15 M and 1.58-3.86 M, respectively. The precision for migration times and peak areas was below 0.56% and 3.74%, respectively. The data proved that the concentration of cations in cells can be accurately quantified. It was found that the K+ concentration decreased from 82.2 M to 52.7 M, and the Na+ concentration increased from 62.4 M to 127.2 M during the process of apoptosis when the cell density was 1 x 10(5) cells per mL. The low-cost CE-(CD)-D-4 provides a convenient way to decipher the interaction of Na+ and K+ in the regulation of cell apoptosis.
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