Journal
RSC ADVANCES
Volume 8, Issue 23, Pages 12785-12790Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8ra01274a
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Funding
- Science and Technology Development Project of the Jilin Province [20170204038GX]
- National Natural Science Foundation of China [21561162004]
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A ratiometric fluorescence assay for acetylcholinesterase activity is established, which is based on controlled perylene probe assembly and monomer-excimer transition. In a buffer solution, a perylene probe with two negatively charged groups (PDI-DHA) mainly exists in monomeric form. In the presence of cationic lauroylcholine and lauric acid, PDI-DHA can form supramolecular assemblies and the perylene excimer emission can be observed. AChE can catalyze the hydrolysis of lauroylcholine to anionic lauric acid and choline. The hydrolysis process can trigger the breakdown of the supramolecular assemblies. The perylene excimer recovers to the monomeric form because of the de-aggregation of the probe. The excimer-monomer transition can be detected, and a ratiometric fluorescence assay for AChE activity and inhibitor screening is therefore established.
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