Journal
JOURNAL OF BIOMEDICAL SCIENCE
Volume 19, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1423-0127-19-58
Keywords
Probiotic; Epithelial cells; Gene expression; Signal transduction genes; Candidiasis; Estrogen
Categories
Funding
- Food and Drug Administration of the Public Health Service
Ask authors/readers for more resources
Background: Vaginal epithelial cells have receptors, signal transduction mechanisms, and cytokine secretion capabilities to recruit host defenses against Candida albicans infections. This research evaluates how probiotic lactobacilli affect the defensive epithelial response. Methods: This study used quantitative reverse transcription-polymerase chain reaction assay (qRT-PCR), flow cytometry, and a multiplex immunoassay to observe changes in the regulation of gene expression related to cytokine responses in the VK2 (E6/E7) vaginal epithelial cell line treated with 17 beta-estradiol, exposed to probiotic Lactobacillus rhamnosus GR-1 (R) and Lactobacillus reuteri RC-14 (R) and challenged with C. albicans. Data were statistically evaluated by repeated measures analysis of variance and paired t-tests where appropriate. Results: C. albicans induced mRNA expression of genes related to inflammatory cytokine responses associated with nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPK) signal transduction pathways. 17 beta-estradiol suppressed expression of interleukin-1 alpha (IL-1 alpha), IL-6, IL-8, and tumor necrosis factor alpha (TNF alpha) mRNA. Probiotic lactobacilli suppressed C. albicans-induced nuclear factor-kappa B inhibitor kinase kinase alpha (I kappa kappa alpha), Toll-like receptor-2 (TLR2), TLR6, IL-8, and TNF alpha, also suggesting inhibition of NF-kappa B signaling. The lactobacilli induced expression of IL-1a, and IL-1 beta mRNA, which was not inhibited by curcumin, suggesting that they induce an alternate inflammatory signal transduction pathway to NF-kappa B, such as the mitogen activated protein kinase and activator protein-1 (MAPK/AP-1) signal transduction pathway. Curcumin inhibited IL-13 secretion, suggesting that expression of this cytokine is mainly regulated by NF-kappa B signaling in VK2 cells. Conclusions: The results suggest that C. albicans infection induces pro-inflammatory responses in vaginal epithelial cells, and estrogen and lactobacilli suppress expression of NF-kappa B-related inflammatory genes. Probiotic lactobacilli may induce IL-1 alpha and IL-1 beta expression by an alternate signal transduction pathway, such as MAPK/AP-1. Activation of alternate signaling mechanisms by lactobacilli to modify epithelial cell cytokine production may be a mechanism for probiotic modulation of morbidity in vulvovaginal candidiasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available