Journal
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
Volume 40, Issue 6, Pages 835-839Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S1872-2040(11)60552-5
Keywords
Carbon nanotubes, Ionic liquid; Alkaline phosphatase; p-Nitrophenol, p-Nitrophenyl phosphate; Clenbuterol
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Funding
- National High-Tech Research and Development Program of China [2009ZX08012-004B]
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An electrochemical method to continuously detect alkaline phosphatase (AP) was developed. Using home-made ionic liquid modified carbon nanotubes electrode (CNTs-ILE), on the basis of the characteristics of CNTs-ILE and the substrate p-nitrophenyl phosphate (PNPP) and product p-nitrophenol (PNP), the continuous detection of the process of AP enzymatic reaction with amperometry was achieved. The results indicated that the cyclic voltammetry feature of the CNTs-ILE was superior to conventional glassy carbon electrode, also a good anti-passivation characteristic was achieved for the detection of PNP. When the concentration of AP was in the range of 1.0-100 U L-1, the current showed a good linear relationship to the concentrations of AP. This method was quick to detect AP, and detection time was less than 20 min.
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