Journal
NUTRIENTS
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/nu10060797
Keywords
goat milk; antioxidant peptides; Lactobacillus casei L61; response surface methodology; simulated gastrointestinal digestion; scavenging rate of hydroxyl radical; DPPH
Categories
Funding
- key project of Science and Technology Department of Shaanxi Province [2018ZDXM-NY-085]
- Science and Technology Project of Weiyang District, Xi'an City [201831]
- Shaanxi Provincial Education Department [17JF005]
- Science and Technology Project of Xianyang city [2017K02-69]
Ask authors/readers for more resources
Antioxidant peptides are currently the focus of many studies, since they eliminate free radicals in the human body without harmful effects. In the present study, Lactobacillus casei L61 was used as a starter culture to ferment goat milk because of its high capacity to produce antioxidant peptides. An optimal nutrients formula (casein, casein peptone, glucose, soybean peptone, inulin, calcium lactate, and cysteine) was investigated by Plackett-Burman (P-B) and Box-Behnken (B-B) designs for response surface methodology (RSM). Antioxidant peptides were successively isolated and purified from the fermented goat milk. Furthermore, the stability of the antioxidant peptides was evaluated in a simulated gastrointestinal tract at 37 degrees C. The results showed that calcium lactate, glucose, and casein peptone significantly affected the antioxidant activity of goat milk. The optimal additive amounts were 0.99% (w/v) calcium lactate, 0.21% (w/v) glucose, and 0.29% (w/v) casein peptone. The hydroxyl free radical scavenging rate increased significantly (p < 0.001) from 56.50 +/- 0.57% to 88.01 +/- 0.69%; the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate increased up to 63.48 +/- 1.22% under the optimal conditions (n = 3). Our research provides a fitted mathematical model for antioxidant peptides production. Besides, these antioxidant peptides had great stability during simulated gastrointestinal digestion.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available