Journal
NUTRIENTS
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/nu10060689
Keywords
apoptosis; endoplasmic reticulum stress; kidney; puromycin aminonucleoside; taurine; ultrastructure
Categories
Funding
- New Pet Food Italia S.r.l. (Italy)
- University of Brescia (Italy)
- Italian Ministry of University and Scientific Research (MIUR)-Finanziamento delle Attivita Base di Ricerca (FFABR)
- Marie Sklodowska Curie Innovative Training Networks [MSCA-ITN-2016721236]
- Spanish Ministry of Economy and Competitiveness [SAF 2015-63904-R, SAF2015-71521-REDC, SAF 2015-71521-REDC]
Ask authors/readers for more resources
Taurine (TAU) is a sulfur-containing beta amino acid that is not involved in protein composition and anabolism, conditionally essential in mammals provided through diet. Growing evidence supports a protective role of TAU supply in osmoregulation, calcium flux, and reduction of inflammation and oxidant damage in renal diseases like diabetes. Endoplasmic reticulum (ER) stress, due to abnormal proteostasis, is a contributor to nephrotic syndrome and related renal damage. Here, we investigated the effect of dietary TAU (1.5% in drinking water for 15 days) in an established rat model that mimics human minimal change nephrosis, consisting of a single puromycin aminonucleoside (PAN) injection (intraperitoneally 15 mg/100 g body weight), with sacrifice after eight days. TAU limited proteinuria and podocytes foot processes effacement, and balanced slit diaphragm nephrin and glomerular claudin 1 expressions. In cortical proximal tubules, TAU improved lysosomal density, ER perimeter, restored proper ER-mitochondria tethering and mitochondrial cristae, and decreased inflammation. Remarkably, TAU downregulated glomerular ER stress markers (GRP78, GRP94), pro-apoptotic C/EBP homologous protein, activated caspase 3, tubular caspase1, and mitochondrial chaperone GRP75, but maintained anti-apoptotic HSP25. In conclusion, TAU, by targeting upstream ER stress separate from mitochondria dysfunctions at crucial renal sites, might be a promising dietary supplement in the treatment of the drug-resistant nephrotic syndrome.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available