Reduced Graphene Oxide as the Support for Lipid Bilayer Membrane

Supported lipid membranes of dioleoylphosphatidylcholine (DOPC) were formed on reduced graphene oxide (r-GO) by the vesicle fusion method. Graphene oxide prepared by chemical exfoliation was deposited on a thermally oxidized SiO2/Si substrate, and was reduced by hydrazine vapour. Flat lipid membranes were formed on the r-GO surface after the r-GO/SiO2/Si substrate was incubated in the DOPC vesicle suspension. We propose a structural model in which a lipid bilayer and monolayer stacked on r-GO based on the results of fluorescence microscope and atomic force microscope observation.