Journal
DRUG DESIGN DEVELOPMENT AND THERAPY
Volume 12, Issue -, Pages 1303-1310Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/DDDT.S149307
Keywords
colorectal cancer; Vicenin-2; beta-catenin; apoptosis; caspase-3
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Background: Colorectal cancer (CRC) is among highest prevailing cancers in the whole world, especially in western countries. For a diverse of reasons, patients prefer naturally occurring dietary substances over synthetic agents to prevent cancer. Vicenin-2 is largely available in a medicinal plant Ocimum sanctum and is an apigenin form, 6,8-di-C-glucoside, which has been reported to have a range of pharmacological values which includes antioxidant, hepatoprotective, anti-inflammatory and anti-cancer. This study was aimed to analyze the anti-proliferative effect of Vicenin-2 on human colon cancer cells via the Wnt/beta-catenin signaling inhibition Methods: MTT assay was used to assess the cell viability at different concentrations and time point. Vicenin-2 at a concentration of 50 mu M (IC50) decreased the phosphorylated (inactive) glycogen synthase kinase-3 beta, cyclin D1, and non-p-beta-catenin expressions in HT-29 cells, which were evidenced through western blot analysis. Results: Further, Vincenin-2 reduced the T-cell factor (TCF)/Leukocyte erythroid factor (LEF) reporter activity in HT-29 cells. Vicenin-2 also promoted substantial cell cycle arrest at the G2M phase of HT-29 cells, as well induced apoptosis in HT-29 cells, as revealed through flow cytometric analysis. Furthermore, immunoblot analysis showed that Vicenin-2 treatment enhanced the expression of Cytochrome C, Bax and caspase-3 whereas suppressed the Bcl-2 expression. Conclusion: Together, these results revealed that Vicenin-2 can act as a potent inhibitor of HT-29 cell proliferation and can be used as an agent against CRC.
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