Colorimetric detection of Cu2+ peptide-copper complexes based on the formation of peptide-copper complexes on silver nanoparticle surfaces

We developed a colorimetric method for the rapid detection of copper ions (Cu2+) in aqueous solution. The detection of Cu2+ is based on coordination reactions of Cu2+ with casein peptide-functionalized silver nanoparticles (AgNPs), leading to a distinct color change of the solution from yellow to red. The developed method has a good detection limit of about 0.16 mu M Cu2+ using 0.05 mL of AgNPs stock solution and a linearity in the range of 0.08-1.44 mu M Cu(2+ )with a correlation coefficient of R-2 = 0.973. The developed method is a useful tool for the detection of Cu2+ ions. Furthermore, it can be used for monitoring Cu2+ in water at concentrations below the safe limit for drinking water set by the World Health Organization.