Journal
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
Volume 10, Issue 4, Pages 406-419Publisher
KOREAN ACAD ASTHMA ALLERGY & CLINICAL IMMUNOLOGY
DOI: 10.4168/aair.2018.10.4.406
Keywords
Dendritic cells; asthma; allergens; house dust mites; immunotherapy and tolerance induction
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Funding
- Programa de Excelencia em Pesquisa (PROEP-CNPq)
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Purpose: The use of tolerogenic dendritic cells (ToIDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of ToIDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods: Mouse bone marrow-derived ToIDCs were induced by incubation with granulocyte-macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme-linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed ToIDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results: Induction of ToIDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed ToIDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, ToIDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-gamma levels in the supernatant of splenocyte cultures Transplantation of ToIDCs increased the percentage of the regulatory T cells in the spleen and the lungs. Conclusions: Preventive treatment with ToIDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.
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