Journal
ACS COMBINATORIAL SCIENCE
Volume 20, Issue 5, Pages 261-268Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscombsci.7b00190
Keywords
Vibrio fischeri; ssDNA aptamer; aptamer-based paper strip sensor
Funding
- Rural Development Administration, Republic of Korea [PJ012568]
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2015R1A4A1041869]
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Aptamer-based paper strip sensor for detecting Vibrio fischeri was developed. Our method was based on the aptamer sandwich assay between whole live cells, V. fischeri and DNA aptamer probes. Following 9 rounds of Cell-SELEX and one of the negative-SELEX, V. fischeri Cell Aptamer (VFCA)-02 and -03 were isolated, with the former showing approximately 10-fold greater avidity (in the subnanomolar range) for the target cells when arrayed on a surface. The colorimetric response of a paper sensor based on VFCA-02 was linear in the range of 4 X 10(1) to 4 X 10(5) CFU/mL of target cell by using scanning reader. The linear regression correlation coefficient (R-2) was 0.9809. This system shows promise for use in aptamer-conjugated gold nanoparticle probes in paper strip format for in-field detection of marine bioindicating bacteria.
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