Journal
ACS CATALYSIS
Volume 8, Issue 4, Pages 3358-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscatal.7b03652
Keywords
biohybrid catalysis; protein engineering; beta-barrel proteins; Grubbs-Hoveyda-type catalysts; olefin metathesis
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Funding
- Deutsche Forschungsgemeinschaft (DFG) through the International Research Training Group Selectivity in Chemo- and Biocatalysis (SeleCa)
- Bundesministerium fur Bildung und Forschung (BMBF) [FKZ 031B0297]
- JSPS KAKENHI [JP1SKT0144, JP17H05370, JP15H05804]
- Interactive Material Science Cadet program (IMSC)
- Umicore, Frankfurt
- Grants-in-Aid for Scientific Research [15H05804] Funding Source: KAKEN
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Incorporation of a synthetic metal catalyst into a protein scaffold yields a biohybrid catalyst, with a remarkable performance in aqueous media and the broad reaction scope of organometallic catalysts. A major challenge for efficient catalysis is the design of the interface between the protein scaffold and the metal catalyst. Until now, protein scaffolds have primarily been engineered by exchanging individual amino acids to anchor metal catalysts and alter their immediate environment. Here, cavity size engineering of the beta-barrel protein nitrobindin was performed by duplicating multiple beta-strands to generate an expanded variant. The approach of cavity size engineering enabled covalent incorporation of bulky catalysts at excellent coupling efficiencies and yielded excellent conversions in olefin metathesis, including ring-closing metathesis, ring-opening metathesis polymerization, and cross metathesis (conversions up to 99% and turnover numbers up to 10000).
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