Related references
Note: Only part of the references are listed.The Essential Genome of Escherichia coli K-12
Emily C. A. Goodall et al.
MBIO (2018)
Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
Andrew D. Garst et al.
NATURE BIOTECHNOLOGY (2017)
CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
S. John Liu et al.
SCIENCE (2017)
RNA targeting with CRISPR-Cas13
Omar O. Abudayyeh et al.
NATURE (2017)
Cas9-mediated genome editing in the methanogenic archaeon Methanosarcina acetivorans
Dipti D. Nayak et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)
Functional Constraints on Replacing an Essential Gene with Its Ancient and Modern Homologs
Betul Kacar et al.
MBIO (2017)
Efficient Transcriptional Gene Repression by Type V-A CRISPR-Cpf1 from Eubacterium eligens
Seong Keun Kim et al.
ACS SYNTHETIC BIOLOGY (2017)
Maltose Utilization as a Novel Selection Strategy for Continuous Evolution of Microbes with Enhanced Metabolite Production
Shu-De Liu et al.
ACS SYNTHETIC BIOLOGY (2017)
Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis
Byoung-Mo Koo et al.
CELL SYSTEMS (2017)
Co-infecting microorganisms dramatically alter pathogen gene essentiality during polymicrobial infection
Carolyn B. Ibberson et al.
NATURE MICROBIOLOGY (2017)
RegulonDB version 9.0: high-level integration of gene regulation, coexpression, motif clustering and beyond
Socorro Gama-Castro et al.
NUCLEIC ACIDS RESEARCH (2016)
Multiple Gene Repression in Cyanobacteria Using CRISPRi
Lun Yao et al.
ACS SYNTHETIC BIOLOGY (2016)
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria
Jason M. Peters et al.
CELL (2016)
Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli
Mingyue Fang et al.
METABOLIC ENGINEERING (2016)
Enhanced Efflux Activity Facilitates Drug Tolerance in Dormant Bacterial Cells
Yingying Pu et al.
MOLECULAR CELL (2016)
Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes
David W. Morgens et al.
NATURE BIOTECHNOLOGY (2016)
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
John G. Doench et al.
NATURE BIOTECHNOLOGY (2016)
CRMAGE: CRISPR Optimized MAGE Recombineering
Carlotta Ronda et al.
SCIENTIFIC REPORTS (2016)
Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase
Tao Xu et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2015)
Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
Yu Jiang et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2015)
Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis
Li Lv et al.
METABOLIC ENGINEERING (2015)
Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium
M. Lluch-Senar et al.
Molecular Systems Biology (2015)
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
Benjamin P. Kleinstiver et al.
NATURE (2015)
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann et al.
NATURE (2015)
Massively parallel high-order combinatorial genetics in human cells
Alan S. L. Wong et al.
NATURE BIOTECHNOLOGY (2015)
High-throughput functional genomics using CRISPR-Cas9
Ophir Shalem et al.
NATURE REVIEWS GENETICS (2015)
Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons
Kelly M. Wetmore et al.
MBIO (2015)
Gene silencing by CRISPR interference in mycobacteria
Eira Choudhary et al.
NATURE COMMUNICATIONS (2015)
Genome-Wide Tuning of Protein Expression Levels to Rapidly Engineer Microbial Traits
Emily F. Freed et al.
ACS SYNTHETIC BIOLOGY (2015)
Toxin YafQ increases persister cell formation by reducing indole signalling
Ying Hu et al.
ENVIRONMENTAL MICROBIOLOGY (2015)
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Luke A. Gilbert et al.
CELL (2014)
The Precarious Prokaryotic Chromosome
Andrei Kuzminov
JOURNAL OF BACTERIOLOGY (2014)
CRISPR-Cas systems for editing, regulating and targeting genomes
Jeffry D. Sander et al.
NATURE BIOTECHNOLOGY (2014)
A Comprehensive tRNA Deletion Library Unravels the Genetic Architecture of the tRNA Pool
Zohar Bloom-Ackermann et al.
PLOS GENETICS (2014)
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Lei S. Qi et al.
CELL (2013)
DNA targeting specificity of RNA-guided Cas9 nucleases
Patrick D. Hsu et al.
NATURE BIOTECHNOLOGY (2013)
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Wenyan Jiang et al.
NATURE BIOTECHNOLOGY (2013)
Engineering central metabolic modules of Escherichia coli for improving β-carotene production
Jing Zhao et al.
METABOLIC ENGINEERING (2013)
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
David Bikard et al.
NUCLEIC ACIDS RESEARCH (2013)
A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium
Lars Barquist et al.
NUCLEIC ACIDS RESEARCH (2013)
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
James E. DiCarlo et al.
NUCLEIC ACIDS RESEARCH (2013)
Integrated platform for genome-wide screening and construction of high-density genetic interaction maps in mammalian cells
Martin Kampmann et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong et al.
SCIENCE (2013)
Sequence-Defined Transposon Mutant Library of Burkholderia thailandensis
Larry A. Gallagher et al.
MBIO (2013)
Signaling-mediated bacterial persister formation
Nicole M. Vega et al.
NATURE CHEMICAL BIOLOGY (2012)
FLASH: fast length adjustment of short reads to improve genome assemblies
Tanja Magoc et al.
BIOINFORMATICS (2011)
Phenotypic Landscape of a Bacterial Cell
Robert J. Nichols et al.
CELL (2011)
The essential genome of a bacterium
Beat Christen et al.
MOLECULAR SYSTEMS BIOLOGY (2011)
Activation of gene expression by small RNA
Kathrin S. Froehlich et al.
CURRENT OPINION IN MICROBIOLOGY (2009)
Update on the Keio collection of Escherichia coli single-gene deletion mutants
Natsuko Yamamoto et al.
MOLECULAR SYSTEMS BIOLOGY (2009)
Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms
Tim van Opijnen et al.
NATURE METHODS (2009)
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson et al.
NATURE METHODS (2009)
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
Carola Engler et al.
PLOS ONE (2009)
SeqMap: mapping massive amount of oligonucleotides to the genome
Hui Jiang et al.
BIOINFORMATICS (2008)
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
Tomoya Baba et al.
MOLECULAR SYSTEMS BIOLOGY (2006)
The modified wobble nucleoside uridine-5-oxyacetic acid in tRNAcmo5UGGPro promotes reading of all four proline codons in vivo
SJ Näsvall et al.
RNA (2004)
Experimental determination and system level analysis of essential genes in Escherichia coli MG1655
SY Gerdes et al.
JOURNAL OF BACTERIOLOGY (2003)
Statistical significance for genomewide studies
JD Storey et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)
PCR-mediated gene replacement in Escherichia coli
KC Murphy et al.
GENE (2000)
Share Paper
