Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-017-02772-x
Keywords
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Categories
Funding
- Common Fund of the Office of the Director of the National Institutes of Health
- US NIH [HHSN261200800001E]
- NCI
- NDRI [10XS170]
- Roswell Park Cancer Institute [10XS171]
- Science Care Inc. [10 x 172]
- IDOX [12ST1039]
- Van Andel Institute [10ST1035]
- Broad Institute [HHSN268201000029C]
- U Miami Brain Bank [R01 DA006227-17]
- Portuguese Foundation for Science and Technology (FCT)
- FEDER funds through the COMPETE-Operacional Programme for Competitiveness and Internationalization (POCI), Portugal
- FCT/MCTES [POCI-01-0145-FEDER-007274]
- NORTE, under the PORTUGAL Partnership Agreement, through the European Regional Development Fund (ERDF) [NORTE-01-0145-FEDER-000029]
- FCT [SFRH/BPD/89764/2012, PD/BD/128007/2016]
- POPH-QREN Type 4.2
- European Social Fund
- MCTES, program Investigador FCT [IF/01127/2014]
- Spanish Ministry of Economy and Competitiveness [BIO2014-57291-R]
- Plataforma de Recursos Biomoleculares y Bioinformaticos from the ISCIII [PT13/0001/0007]
- EU ELIXIR-EXCELERATE Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa' [6,676559]
- CERCA Programme/Generalitat de Catalunya
- Ministerio de Educacion, Cultura y Deporte, under the FPU programme (Formacion de Profesorado Universitario) [FPU15/03635]
- National Human Genome Research Institute [1R01MH101814, 3R01MH101814-03S1]
- European Research Council under the European Union's Seventh Framework Programme / ERC [RNA-MAPS_294653]
- Ministerio de Economia, Industria y Competitividad, y los Fondos Estructurales de la Union Europea (FEDER) [MEIC BIO2015-70777-P]
- ISCIII del Ministerio de Economia y Competitividad, cofinaciada por el Fondo Europeo de Desarrollo Regional (FEDER) [PT13/0001/0021]
- CNPq [310132/2015-0, 427541/2016-6]
- FAPERJ [E_06/2015]
- Fundação para a Ciência e a Tecnologia [PD/BD/128007/2016, SFRH/BPD/89764/2012] Funding Source: FCT
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Post-mortem tissues samples are a key resource for investigating patterns of gene expression. However, the processes triggered by death and the post-mortem interval (PMI) can significantly alter physiologically normal RNA levels. We investigate the impact of PMI on gene expression using data from multiple tissues of post-mortem donors obtained from the GTEx project. We find that many genes change expression over relatively short PMIs in a tissue-specific manner, but this potentially confounding effect in a biological analysis can be minimized by taking into account appropriate covariates. By comparing ante-and postmortem blood samples, we identify the cascade of transcriptional events triggered by death of the organism. These events do not appear to simply reflect stochastic variation resulting from mRNA degradation, but active and ongoing regulation of transcription. Finally, we develop a model to predict the time since death from the analysis of the transcriptome of a few readily accessible tissues.
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