Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-03533-0
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Funding
- Chica and Heinz Schaller Foundation
- Deutsche Forschungsgemeinschaft (DFG) [SFB1129]
- Hartmut Hoffmann-Berling International Graduate School of Molecular & Cellular Biology (HBIGS) at the Heidelberg University
- Boehringer Ingelheim Fonds
- Heidelberg Graduate School of Fundamental Physics (HGSFP) at the Heidelberg University
- National Heart Lung and Blood Institute, National Institutes of Health, USA
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [ZIAHL006098] Funding Source: NIH RePORTER
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Although essential for many cellular processes, the sequence of structural and molecular events during clathrin-mediated endocytosis remains elusive. While it was long believed that clathrin-coated pits grow with a constant curvature, it was recently suggested that clathrin first assembles to form flat structures that then bend while maintaining a constant surface area. Here, we combine correlative electron and light microscopy and mathematical growth laws to study the ultrastructural rearrangements of the clathrin coat during endocytosis in BSC-1 mammalian cells. We confirm that clathrin coats initially grow flat and demonstrate that curvature begins when around 70% of the final clathrin content is acquired. We find that this transition is marked by a change in the clathrin to clathrin-adaptor protein AP2 ratio and that membrane tension suppresses this transition. Our results support the notion that BSC-1 mammalian cells dynamically regulate the flat-to-curved transition in clathrin-mediated endocytosis by both biochemical and mechanical factors.
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