Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-03926-1
Keywords
-
Categories
Funding
- Netherlands Organization for Scientific Research (NWO/OCW) as part of the Frontiers of the Nanoscience Program
- Netherlands Organization for Scientific Research (NWO/OCW) through a VIDI grant [723.012.007]
- Spanish Ministry of Economy and Competitiveness [BFU2014-52656-P]
Ask authors/readers for more resources
Replication of DNA-encoded information and its conversion into functional proteins are universal properties of life. In an effort toward the construction of a synthetic minimal cell, we implement here the DNA replication machinery of the F29 virus in a cell-free gene expression system. Amplification of a linear DNA template by self-encoded, de novo synthesized F29 proteins is demonstrated. Complete information transfer is confirmed as the copied DNA can serve as a functional template for gene expression, which can be seen as an autocatalytic DNA replication cycle. These results show how the central dogma of molecular biology can be reconstituted and form a cycle in vitro. Finally, coupled DNA replication and gene expression is compartmentalized inside phospholipid vesicles providing the chassis for evolving functions in a prospective synthetic cell relying on the extant biology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available