Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-03149-4
Keywords
-
Categories
Funding
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Wellcome Trust
- EU Blueprint
- EpiGeneSys
- BBSRC
- Medical Research Council (MRC)
- European Molecular Biology Laboratory (EMBL)
- EU
- EPSRC Centre for Doctoral Training in Data Science [EP/L016427/1]
- University of Edinburgh
- Wellcome Trust [105031/Z/14/Z] Funding Source: Wellcome Trust
- BBSRC [BBS/E/B/000C0422, 1645504, BBS/E/B/000C0426, BBS/E/B/0000H334] Funding Source: UKRI
- MRC [MR/K011332/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/B/0000H334, 1645504, BBS/E/B/000C0422, BBS/E/B/000C0426] Funding Source: researchfish
- Cancer Research UK [22231] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1497322] Funding Source: researchfish
- Medical Research Council [MR/K011332/1] Funding Source: researchfish
- Wellcome Trust [105031/Z/14/Z] Funding Source: researchfish
Ask authors/readers for more resources
Parallel single-cell sequencing protocols represent powerful methods for investigating regulatory relationships, including epigenome-transcriptome interactions. Here, we report a single-cell method for parallel chromatin accessibility, DNA methylation and transcriptome profiling. scNMT-seq (single-cell nucleosome, methylation and transcription sequencing) uses a GpC methyltransferase to label open chromatin followed by bisulfite and RNA sequencing. We validate scNMT-seq by applying it to differentiating mouse embryonic stem cells, finding links between all three molecular layers and revealing dynamic coupling between epigenomic layers during differentiation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
