Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-04116-9
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Funding
- Helmholtz Association through project-oriented funds
- Hamburg Center of Ultrafast Imaging, University of Hamburg
- Human Frontiers Science Program [RGP0010/ 2017]
- U.S. Department of Energy by Lawrence Livermore National Laboratory [DE-AC52-07NA27344]
- LLNL Lab-directed Research and Development (LDRD) [12-ERD-031]
- NIH [1R01GM117342-01]
- European Research Council under the European Union's Seventh Framework Programme ERC Synergy Grant [609920]
- New Zealand Marsden Grant
- Wellcome (studentship) [ALR00040]
- Lundbeck Foundation BRAINSTRUC Initiative
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM117342] Funding Source: NIH RePORTER
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Here we present a new approach to diffraction imaging of amyloid fibrils, combining a freestanding graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 A and 2.4 A resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.
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