Journal
JOURNAL OF PLANT INTERACTIONS
Volume 13, Issue 1, Pages 329-337Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/17429145.2018.1478994
Keywords
Drought tolerance; Malus baccata; WRKY transcription factor; MbWRKY3; transformed tobacco
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Funding
- National Natural Science Foundation of China [31301757]
- Natural Science Foundation of Heilongjiang Province [C2015015]
- Academic Backbone Project of Northeast Agricultural University [15XG06]
- Postdoctoral Scientific Research Development Fund of Heilongjiang Province, China [LBH-Q16020]
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Plant-specific WRKY transcription factors were involved in stress responses and ABA signaling. In the present study, a WRKY gene is isolated from Malus baccata (L.) Borkh and designated as MbWRKY3. Subcellular localization revealed that MbWRKY3 was localized onto nucleus. The MbWRKY3 expression levels were up-regulated by salinity, drought, and ABA treatments in M.baccata. When MbWRKY3 was introduced into tobaccos, it improved drought stress tolerance in transgenic plants. Compared to WT, the transgenic tobaccos had the higher levels of relative water content, and proline and chlorophyll contents, decreased levels of electrolyte leakage, MDA, and H2O2, increased activities of the reactive oxygen species-related enzymes (SOD, CAT, and POD), and greater up-regulations of the corresponding genes (NtSOD, NtCAT, and NtPOD), especially when dealt with drought stress. These results suggest that MbWRKY3 gene plays a positive regulatory role in drought stress response.
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