Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 42, Issue -, Pages 21-29Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2018.01.008
Keywords
C-phycocyanin; Anti-inflammation; Dynamic proteomics; Programmed cell death 5 protein; NF-kappa B pathway
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Funding
- National Natural Science Foundation of China (NSFC) [31701575, 31571801]
- Beijing Excellent Talents Training Project [2016000020124G025]
- National Key Research and Development Program [2016YFD0400502-02]
- Beijing Municipal Science and Technology Project [Z171100002217019]
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C-phycocyanin (C-PC), a type of functional food colourant, plays a significant role in regulating inflammatory responses. However, its underlying anti-inflammatory mechanism remains unknown. Previously, we have established a S-35 in vivo/vitro labelling analysis for dynamic proteomic (SiLAD) technology. Unlike traditional proteomic methods, SiLAD exclusively detects synthesis rates instead of accumulated amounts through 35S pulse labelling of newly synthesized proteins, providing a high sensitive way for analysing protein alterations. In this work, we applied SiLAD to explore the anti-inflammatory mechanism of C-PC. We found that the protein synthesis rates were specifically detected and visualised. Strikingly, network analysis suggested programmed cell death 5 protein (PDCD5) was involved in C-PC mediated anti-inflammatory process. Phenotyping experiments demonstrated that C-PC could reduce inflammation through down-regulating PDCD5-NF-kappa B signaling. These results provided important information for the regulation of C-PC, and offered a novel perspective for regulatory mechanism of functional food.
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