Journal
BIOANALYSIS
Volume 10, Issue 6, Pages 377-384Publisher
FUTURE SCI LTD
DOI: 10.4155/bio-2017-0228
Keywords
acute kidney injury; cystatin-C; tubular damage; turbidimetry; urine; validation
Funding
- University of Pecs, Medical School [KA-2016-04]
- 'New National Excellence Program of the Ministry of Human Capacities' grants [UNKP-17-3-III]
- [NKFI-EPRK/115394/2015]
- [GINOP-2.3.2-15-2016-00021]
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Aim: There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. Materials & methods: A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed. Results: Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes. Conclusion: We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.
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