Functionalization of Strongly Interacting Magnetic Nanocubes with (Thermo)Responsive Coating and Their Application in Hyperthermia and Heat-Triggered Drug Delivery

Herein, we prepare nanohybrids by incorporating iron oxide rianocubes (cubic-IONPs) within a thermoresponsive polymer shell that can act as drug carriers for doxorubicin(doxo). The cubic-Shaped nanoparticles employed are at the interface between superparamagnetic and ferromagnetic behavior and have an exceptionally high specifie absorption rate (SAR), but their functionalization is extremely challenging compared to bare isuperparamagnetic iron oxide nanopartides as they strongly interact with each other. By conducting the polymer grafting reaction using reversible addition fragmentation chain transfer.(RAFT) polymerization in a viscous solvent medium, we have here developed a. facile approach to decorate the nanocubes with stimuli-responsive polymers. When the thermoresponsive shell is composed of poly(Nisopropylacrylamide-copolyethylene glycolmethyl ether acrylate), nanohybtids have a phase transition temperature, the lower critical solution temperature (LCST), above 37 degrees C m physiological conditions. DOM), loaded nanohybtids exhibited a negligible drug release below 37 degrees C but showed a consistent release of their cargo on demand bY exploiting the capability of the nanocubeS to generate heat under an alternating magnetic field (AMF). Moreover, the drug free nanocarrier does not exhibit cytotoxicity even when administered at high concentration of nanocubes (lg/L of iron) and internalized at high extent (260 pg of iron per cell). We have also implemented the synthesis protocol to decorate the surface of nanocubes with poly(vinylpyridine) polymer and thus prepare pH-responsive shell coated nano-cubes.