Journal
METABOLITES
Volume 2, Issue 3, Pages 496-515Publisher
MDPI
DOI: 10.3390/metabo2030496
Keywords
capillary electrophoresis; gas chromatography; high performance liquid chromatography; highly polar metabolite
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Funding
- MEXT at Private Universities
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Metabolomics is a new approach that is based on the systematic study of the full complement of metabolites in a biological sample. Metabolomics has the potential to fundamentally change clinical chemistry and, by extension, the fields of nutrition, toxicology, and medicine. However, it can be difficult to separate highly polar compounds. Mass spectrometry (MS), in combination with capillary electrophoresis (CE), gas chromatography (GC), or high performance liquid chromatography (HPLC) is the key analytical technique on which emerging omics technologies, namely, proteomics, metabolomics, and lipidomics, are based. In this review, we introduce various methods for the separation of highly polar metabolites.
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