Journal
NORTH AMERICAN JOURNAL OF MEDICAL SCIENCES
Volume 4, Issue 9, Pages 429-434Publisher
MEDKNOW PUBLICATIONS & MEDIA PVT LTD
DOI: 10.4103/1947-2714.100998
Keywords
Bio-medical research; protein; western blot
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Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western blot, and offer some ways to troubleshoot.
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