Journal
MOSCOW UNIVERSITY CHEMISTRY BULLETIN
Volume 67, Issue 5, Pages 241-248Publisher
PLEIADES PUBLISHING INC
DOI: 10.3103/S0027131412050045
Keywords
progesterone; lateral flow immunoassay; horseradish peroxidase
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A new express method based on lateral flow immunoassay (LFIA) for progesterone detection was developed. To increase the assay sensitivity an enzyme label (horse-radish peroxidase) was used instead of colloidal gold. An optimal assay format was chosen and the influence of a range of buffer supplements (detergents, proteins and sucrose) was investigated by enzyme-linked immunosorbent assay (ELISA). Linear range of LFIA was between 2 and 40 ng/mL in buffer. Limit of detection was 2 ng/mL, assay time was within 15 min.
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