Journal
VIRUSES-BASEL
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/v10060330
Keywords
lncRNA; influenza A virus; PAAN; polymerase basic protein 1 (PB1); polymerase acidic protein (PA); RdRp
Categories
Funding
- National Key Research and Development program of China [2016YFD0500307]
- National Mega-Project for Infectious Disease [2018ZX10301408]
- National Natural Science Foundation of China [81501756]
- Beijing Key Laboratory of Emerging Infectious Diseases Project
- Xiehe Scholar
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The productive infection of influenza A virus (IAV) depends on host factors. However, the involvement of long non-coding RNAs (lncRNAs) in IAV infection remains largely uninvestigated. In this work, we have discovered a human lncRNA, named lncRNA-PAAN (PA-associated noncoding RNA) that enhances IAV replication. The level of lncRNA-PAAN increases upon infection of IAV, but not other viruses, nor interferon treatment, suggesting specific up-regulation of lncRNA-PAAN expression by IAV. Silencing lncRNA-PAAN significantly decreases IAV replication through impairing the activity of viral RNA-dependent RNA polymerase (RdRp). This function of lncRNA-PAAN is a result of its association with viral PA protein, a key component of IAV RNA polymerase complex. Consequently, depletion of lncRNA-PAAN prevents the formation of functional RdRp. Together, these results suggest that lncRNA-PAAN promotes the assembly of viral RNA polymerase, thus warranting efficient viral RNA synthesis. Elucidating the functions of lncRNAs in IAV infection is expected to advance our understanding of IAV pathogenesis and open new avenues to the development of novel anti-IAV therapeutics.
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