4.6 Article

Identification of Ellagic Acid from Plant Rhodiola rosea L. as an Anti-Ebola Virus Entry Inhibitor

Journal

VIRUSES-BASEL
Volume 10, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/v10040152

Keywords

Ebola; Marburg; Rhodiola rosea; ellagic acid; gallic acid; Traditional Chinese medicine

Categories

Funding

  1. Key R & D project in Shandong Province [2017CXGC1309]
  2. National Major Scientific and Technological Projects Major New Drug Development [2018ZX09711001-003-004]
  3. Key Research and Development Projects of Science and Technology Department of Shandong Province [2016GSF202040]
  4. Shandong Provincial Education Department [J16ML09]
  5. Shandong Province Medical and Health Technology Development Project [2016WSB22003]
  6. NIH [5R21AI115082-02]
  7. NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R21AI115082] Funding Source: NIH RePORTER

Ask authors/readers for more resources

The recent 2014-2016 West African Ebola virus epidemic underscores the need for the development of novel anti-Ebola therapeutics, due to the high mortality rates of Ebola virus infections and the lack of FDA-approved vaccine or therapy that is available for the prevention and treatment. Traditional Chinese medicines (TCMs) represent a huge reservoir of bioactive chemicals and many TCMs have been shown to have antiviral activities. 373 extracts from 128 TCMs were evaluated using a high throughput assay to screen for inhibitors of Ebola virus cell entry. Extract of Rhodiola rosea displayed specific and potent inhibition against cell entry of both Ebola virus and Marburg virus. In addition, twenty commercial compounds that were isolated from Rhodiola rosea were evaluated using the pseudotyped Ebola virus entry assay, and it was found that ellagic acid and gallic acid, which are two structurally related compounds, are the most effective ones. The activity of the extract and the two pure compounds were validated using infectious Ebola virus. The time-of-addition experiments suggest that, mechanistically, the Rhodiola rosea extract and the effective compounds act at an early step in the infection cycle following initial cell attachment, but prior to viral/cell membrane fusion. Our findings provide evidence that Rhodiola rosea has potent anti-filovirus properties that may be developed as a novel anti-Ebola treatment.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available