BioCell print utilizing patterning with electrostatically injected droplet (PELID) method

The objective of this study is to fabricate three-dimensional cell structures utilizing patterning with electrostatically injected droplet (PELID) method, because it is preferable to perform laboratory experiments with 3D cell structures in tissue engineering and artificial organ. However, it is difficult to fabricate 3D cell structures, because own weight of the cell is above the bonding force between cells. In this paper, we printed Madin-Darby canine kidney cells and collagen as scaffolds utilizing the PELID method. We investigated growth of printed cells. Number of printed cells was increased day by day. We investigated the fundamental characteristics on patterning collagen. The printed collagen was thick when the time to print was increased. These results indicated that it is possible to fabricate 3D cell structure.