Journal
VACCINE
Volume 36, Issue 30, Pages 4471-4476Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2018.06.025
Keywords
Tuberculosis; DNA vaccine; Dissolving microneedle; Mice
Categories
Funding
- National Nature Science Foundation of China [31672530]
- Public Technology Research and International Cooperation Projects from Science and Technology Department of Zhejiang Province [2015C34005]
- Natural Science Foundation of Hubei Province [2014CFB230]
- Basic and Advanced technology research projects of Henan Province [162300410091]
- Shenzhen Key Laboratory of Bioship [ZDSYS201504301534057]
- Special Support Funds of Shenzen for introduced High Level Medical Team
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Tuberculosis (TB) remains a major global public health problem. New immunization methods against TB are urgently needed. Plasmid DNA with a microneedle patch is a potentially attractive strategy to improve the immune effect. A DNA vaccine encoding the secreted protein Ag85B of Mycobacterium tuberculosis was immunized in the skin using microneedles, which can improve protective immunity compared to conventional intramuscular (IM) injection. There is no significant difference between microneedle patch (MNP) and IM immunization when the immunizing dose is low (4.2 mu g). However, the results for detecting humoral immunity showed MNP immunization could better provoke an antibody response than IM when the dose is high (12.6 mu g). A similar result was observed in cellular immune responses by measuring the cytokines in splenocytes. The effective protection of MNP can also be demonstrated by counting bacteria and analyzing the survival rate. This study indicated that DNA vaccination in the skin using dissolving microneedles may provide a new strategy against TB. (C) 2018 Elsevier Ltd. All rights reserved.
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