Journal
UPSALA JOURNAL OF MEDICAL SCIENCES
Volume 123, Issue 1, Pages 28-42Publisher
UPSALA MED SOC
DOI: 10.1080/03009734.2018.1431744
Keywords
AMD; complement system; ocular diseases; retina; RPE
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Funding
- Carmen and Bertil Regners Foundation
- Crown Princess Margaretas Committee for the Blind
- Olle Engqvist Foundation
- Sven and Dagmar Salens Foundation
- Ogonfonden ('The Eye Foundation')
- FORSS (Medical Research Council of Southeast Sweden)
- faculty of Medical Sciences at Orebro University
- Linnaeus University
- Swedish Medical Research Council (VR)
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Background: The complement system (CS) plays a role in the pathogenesis of a number of ocular diseases, including diabetic retinopathy (DR), glaucoma, uveitis, and age-related macular degeneration (AMD). Given that many of the complex eye-related degenerative diseases have limited treatment opportunities, we aimed to mimic the in vivo retinal degenerative process by developing a relevant co-culture system. Method and materials: The adult porcine retina was co-cultured with the spontaneously arising human retinal pigment epithelial cells-19 (ARPE-19). Results: Inflammatory activity was found after culture and included migrating microglial cells, gliosis, cell death, and CS activation (demonstrated by a minor increase in the secreted anaphylotoxin C3a in co-culture). CS components, including C1q, C3, C4, soluble C5b-9, and the C5a receptor, were expressed in the retina and/or ARPE cells after culture. C1q, C3, and CS regulators such as C4 binding protein (C4BP), factor H (CFH), and factor I (CFI) were secreted after culture. Discussion: Thus, our research indicates that this co-culturing system may be useful for investigations of the CS and its involvement in experimental neurodegenerative diseases.
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