Journal
ULTRASONICS SONOCHEMISTRY
Volume 40, Issue -, Pages 201-205Publisher
ELSEVIER
DOI: 10.1016/j.ultsonch.2017.07.012
Keywords
Ultrasound; Protease; Bacillus circulans; Casein; Biocatalytic activity
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Funding
- SERB, Department of Science and Technology, New Delhi, India under Fast track Project Scheme [SB/FT/LS-334/2012]
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This study reports the ultrasound-assisted improvements in biocatalytic activity and production of protease from organic solvent-tolerant, alkaliphilic Bacillus circulans MTCC 7942. The strain withstands 10 min ultrasound treatment (33 kHz); this treatment promotes the growth of bacterium. The protease production was improved by 1.5-fold on multiple ultrasound treatment at 0, 6, 12 h intervals. Although the substrate casein remains unaffected by ultrasound treatment (33 kHz, 10 min); the treatment had boosted the enzyme activity of purified protease. Also, the ultrasound treated purified protease showed enhancement in enzyme activity in presence of n-dodecane, n-octane, n-hexadecane, n-decane, xylene, butanol, toluene, pyridine and chloroform.
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