Journal
TRANSLATIONAL RESEARCH
Volume 192, Issue -, Pages 1-14Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.trsl.2017.10.005
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Funding
- Ray W. Poppleton Endowment
- NSF Center funding [EEC-0914790]
- NIH [R21OD017244, R01NS047175, R00 HL121284-01]
- National Center for Research Resources [UL1RR025755, UL1TR001070]
- NCI [P30CA16058]
- NIH Roadmap for Medical Research
- Center for Advanced Functional Foods Research and Entrepreneurship at OSU
- SEED Grant from College of Education and Human Ecology at OSU
- Accelerator Grant from Office for Technology and Commercialization at OSU
- NATIONAL CANCER INSTITUTE [P30CA016058] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UL1TR001070] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [UL1RR025755] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R00HL121284] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS047175, R01NS083942] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [R21OD017244] Funding Source: NIH RePORTER
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Innervation is a fundamental basis for function and survival of tissues. In the peripheral tissues, degenerative diseases create a neurotoxic metabolic milieu that either causes neurodegeneration or fails to sustain regenerative growth and reinnervation of injured/diseased tissues. Encapsulation of cells producing neurotrophic factors can augment axon growth and neuron survival; however, sustained innervation in vivo requires a combination of factors promoting axon growth and guidance pathway that are released in a tissue-specific context. Using novel encapsulation techniques and genetic tools, we manipulated retinoic acid generating enzyme aldehyde dehydrogenase 1 al(Aldhla1) in adipocytes that are capable of promoting growth and innervation of white adipose tissue by sympathetic neurons. Aldhla1(-/-) adipocytes secrete molecules that regulate axon guidance and markedly stimulate neurite outgrowth in vitro and in vivo. Based on studies with natural and synthetic RAR agonists and antagonists, gene microarray and nanostring arrays, we concluded that ephrin A5/ephrin A4 is a downstream pathway regulated by Aldhlal. Encapsulation of Aldhla1(-/-) adipocytes into alginate poly-L-lysine microcapsules induced functional innervation of adipose tissue in obese wild-type mice. We propose that encapsulated Aldhla1(-/-) adipocytes could provide a therapeutic solution for the reinnervation of damaged tissues.
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