Journal
TRAFFIC
Volume 19, Issue 5, Pages 370-379Publisher
WILEY
DOI: 10.1111/tra.12560
Keywords
cargo; COPI; endomembrane; Lam5; SWAT library; yeast
Categories
Funding
- Deutsche Forschungsgemeinschaft [SFB 1190]
- Israel Science Foundation [760/17]
- Mizutani Foundation for Glycoscience
- MRC [MC_U105178783] Funding Source: UKRI
- Medical Research Council [MC_U105178783] Funding Source: researchfish
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A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of similar to 500N-terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co-localizing them with 7 known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact sites between the endoplasmic reticulum and the late Golgi. Additionally, analysis of interactions between the COPI coat and co-localizing proteins from our screen identifies a subset of proteins that are COPI-cargo. In summary, our approach defines the protein roster within each compartment enabling characterization of the physical and functional organization of the endomembrane system and its components.
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