Journal
TOXICOLOGY LETTERS
Volume 292, Issue -, Pages 12-19Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2018.04.019
Keywords
Bisphenol A; Estradiol; Myoblast differentiation; Akt signaling
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Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2017R1D1A1B03032839, NRF-2016R1A2B4014868, NRF-2011-0030074]
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Bisphenol A (BPA), one of the most widespread endocrine disrupting chemicals, is known as an artificial estrogen, which interacts with estrogen receptor (ER). In this study, we investigated the effects of BPA and estradiol on myoblast differentiation and the underlying signaling mechanism. Exposure to BPA (0.01-1 mu M) in mouse myoblast C2C12 cells attenuated myogenic differentiation via the reduced expression of muscle-specific genes, such as myosin heavy chain (MHC), MyoD, and Myogenin, without the alteration of cell proliferation and viability. BPA-exposed C2C12 myoblasts also showed a reduction of Akt phosphorylation ((37-61) %, p < 0.001), a key event for myogenesis. Similarly to BPA, estradiol (0.01-1 mu M) reduced the expression of muscle-specific proteins and the formation of multinucleated myotubes, and attenuated the muscle differentiation- specific phosphorylation of Akt ((42-59) %, p < 0.001). We conclude that BPA and estradiol suppress myogenic differentiation through the inhibition of Akt signaling.
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