Journal
TOXICOLOGY LETTERS
Volume 287, Issue -, Pages 23-30Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2018.01.021
Keywords
Alcohol withdrawal-induced anxiety; Gut microbiota; Intestinal bacterial composition; Faecal microbiota transplantation
Categories
Funding
- National Natural Science Foundation of China [81502664, 81572969, 81402541]
- CAMS Innovation Fund for Medical Sciences (CIFMS) [2016-I2M-1-017]
- Fundamental Research Funds for CAMS/PUMC [2016ZX310200]
- PUMC Youth Fund
- Fundamental Research Funds for the Central Universities [33320140187, 3332016099, 3332016143]
- IRM-CAMS Research Fund [1547, 1522]
- Technology and Development and Research Projects for Research Institutes, Ministry of Science and Technology [2014EG150134]
- Tianjin Science and Technology Support Plan Project (TJKJZC) [14ZCZDSY00001]
- U.S. National Center of Complementary and Alternative Medicine (NCCAM) [R01AT005076]
- National Institute of General Medical Sciences (NIGMS) [R01GM063075]
Ask authors/readers for more resources
Excessive alcohol consumption remains a major public health problem that affects millions of people worldwide. Accumulative experimental evidence has suggested an important involvement of gut microbiota in the modulation of host's immunological and neurological functions. However, it is previously unknown whether enteric microbiota is implicated in the formation of alcohol withdrawal-induced anxiety. Using a murine model of chronic alcoholism and withdrawal, we examined the impact of alcohol consumption on the possible alterations of gut microbiota as well as alcohol withdrawal-induced anxiety and behavior changes. The 16S rRNA sequencing revealed that alcohol consumption did not alter the abundance of bacteria, but markedly changed the composition of gut microbiota. Moreover, the transplantation of enteric microbes from alcohol-fed mice to normal healthy controls remarkably shaped the composition of gut bacteria, and elicited behavioral signs of alcohol withdrawal-induced anxiety. Using quantitative real-time polymerase chain reaction, we further confirmed that the expression of genes implicated in alcohol addiction, BDNF, CRHR1 and OPRM1, was also altered by transplantation of gut microbes from alcohol-exposed donors. Collectively, our findings suggested a possibility that the alterations of gut microbiota composition might contribute to the development of alcohol withdrawal-induced anxiety, and reveal potentially new etiologies for treating alcohol addiction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available