Journal
ACTA NATURAE
Volume 4, Issue 1, Pages 78-81Publisher
RUSSIAN FEDERATION AGENCY SCIENCE & INNOVATION
DOI: 10.32607/20758251-2012-4-1-78-81
Keywords
dynamic light scattering; zeta potential; HeLa cells, MCF-7, mononuclear leukocytes, erythrocytes; apoptosis; phosphatidylserine; membranotropic polymers
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Funding
- Program of Development of Innovative Infrastructure in Kazan (Volga Region) Federal University (Decree of the Government of the Russian Federation) [219]
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The dynamic light scattering (DLS) technique was applied in order to assess the zeta potential of the plasma membrane of human cells. At pH 7.4, the cell zeta potential for different types of cells showed variations over a wide range and was equal to -19.4 +/- 0.8 mV for He La cells and -31.8 +/- 1.1 mV for erythrocytes. The difference could presumably be attributed to the differences in the biochemical composition of the cell plasma membrane. As a result of the heating of He La cells, the zeta potential shifted towards more negative voltages by 4.2 mV. An increase in the zeta potential correlated with an increase in the content of phosphatidylserine on the cell surface, which is considered to be an early marker of apoptosis. The DLS technique was also used to study the interactions between the cells and membranotropic polymers, such as polycations and nonionogenic Pluronic L121.
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