Journal
TALANTA
Volume 184, Issue -, Pages 520-526Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.03.043
Keywords
Solid phase microextraction; In vivo tracing; Salicylic acid; Liquid chromatography-photodiode array detection
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Funding
- National Natural Science Foundation of China [21377172, 21477166, 21527813, 21677182, 21737006]
- NSF of Guangdong Province [S2013030013474]
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In recent years, great concerns have been raised about salicylic acid (SA) and its derivatives as plant regulators. Therefore, precise determination of the distribution of SAs in the living plants is necessary for not only fundamental researches but also the regulating mechanisms. In this study, a custom-made solid phase micro extraction (SPME) fiber based on diallyl dimethyl ammonium chloride-assembled graphene oxide-coated C18 composite (C18@GO@PDDA) was proposed for in vivo detection of salicylic acid, acetylsalicylic acid (ASA), 4 methyl salicylic acid (4-SA) and 3-methyl salicylic acid (3-SA) in aloe plants. Under the optimized conditions, the analytical performance evaluated in homogenized aloe plant tissues exhibited low detection limits (1.8-2.8 mu g g(-1)), wide linear ranges (10-5000 mu g g(-1)), and satisfactory reproducibility (relative standard deviations less than 8.4% and 9.3% for inter-fiber and intra-fiber assays, respectively). Under cadmium stress, the developed method was applied for the in vivo tracing of four salicylic acids in aloe plants. A 48-h in vivo tracing revealed that salicylic acids were involved in the pathway of cadmium stress tolerance. To our best knowledge, it is the first effort to realize the in vivo analysis of SA and its derivatives in plants, and it has a made a great step forward in the area of plant hormone analysis.
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