Journal
TALANTA
Volume 185, Issue -, Pages 113-117Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.03.045
Keywords
Carbon nanosphere; Fluorescence quenching; Aptasensor; Mucin1; MCF-7 cell
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Funding
- State key Basic Research Program of the PRC [2014CB744501]
- NSF of China [81771976, 21475021]
- Fundamental Research Funds for the Central Universities
- Southeast University
- Nanjing Medical University
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In this work, carbon nanosphere (CNS)-based fluorescence turn off/on aptasensor was developed for targeted detection of breast cancer cell MCF-7 by conjugation with FAM (a dye)-labeled mucin1 (MUC1) aptamer P0 (P0-FAM), which can recognize MUC1 protein overexpressed on the surface of MCF-7. Different from other carbon based fluorescence quenching materials, CNSs prepared by the carbonization of glucose not only have the high fluorescence quenching efficiency (98.8%), but also possess negligible cytotoxicity (in the concentration range of 0-1 mg/mL, which is 10 times higher than that of traditional carbon nanotubes or graphene oxide (0-100 mu g/mL)). As for the detection of the mimic of the tumor antigen MUC1, the resulting fluorescence intensity increases nearly linearly in the range of 0-6 mu M with the limit of detection (LOD) of 25 nM.
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