Highly sensitive detection of hepatitis C virus DNA by using a one-donor-four-acceptors FRET probe

Sensitive detection of Hepatitis C virus (HCV) DNA is critical to treat and prevent the propagation of hepatitis C diseases. Herein a FRET probe of One-Donor-Multi-Acceptors (D-mA) assembly is designed through the formation of triple-helix DNA structure, which can greatly reduce the background of FRET system so as to greatly increase the detection sensitivity of hepatitis C virus DNA with the limit of detection (30) being 24.57 nmol/L, making the detection of clinic samples successful and showing that the newly developed FRET probe of D-mA assembly could be applied for the early diagnosis and treatment of hepatitis C disease.