Journal
TALANTA
Volume 182, Issue -, Pages 164-170Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.01.055
Keywords
MALDI-MS; STEC; Mass spectrometry; Spectral processing; Escherichia coli; Shigatoxigenic E. coli
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Funding
- Scottish Government Rural and Environment Science and Analytical Services Division (RESAS)
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Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios.
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