Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 41, Issue 1, Pages 30-37Publisher
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2017.09.004
Keywords
Edwardsiella; AFLP; MLSA; 16S rRNA gene sequence; Taxonomy
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Funding
- Ministerio de Economia y Competitividad (Spain) [AGL2012-31049]
- Xunta de Galicia (Spain) [GRC-2014/007]
- Ministerio de Economia y Competitividad (Spain) for a research fellowship [BES-2010-037413, EEBB-I-13-07222]
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Until 2012, the genus Edwardsiella was composed by three species Edwardsiella tarda, Edwardsiella hoshinae and Edwardsiella ictaluri. In 2013, Edwardsiella piscicida, compiling fish pathogenic strains previously identified as E. tarda was described, and more recently a new species isolated from diseased eel was reported, namely Edwardsiella anguillarum. The incorporation of these species into the genus makes necessary a revision of the taxonomic position of the isolates previously identified as E. tarda. Using AFLP technique, MLSA studies and in silico DNA-DNA hybridization, 46 of 49 E. tarda isolates were re-assigned as E. piscicida and 2 as E. anguillarum, whereas it was confirmed previous classification of the Edwardsiella types and reference strains used. The study of the taxonomic resolution of the genes 16S rRNA, adk, atpD, dnal ginA, hsp60, tuf as well as the possible combinations among housekeeping genes, showed that the gene dnaJ was the more resolutive. In conclusion, the use of molecular techniques is necessary to accurately identify Edwardsiella isolates, especially when differentiating new species from E. tarda. (C) 2017 Elsevier GmbH. All rights reserved.
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