Journal
STEM CELL RESEARCH
Volume 28, Issue -, Pages 61-65Publisher
ELSEVIER
DOI: 10.1016/j.scr.2018.01.020
Keywords
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Funding
- the National Basic Research Program of China (973 program) [2012CB944901]
- National Natural Science Foundation of China [81101510]
- Natural Science Foundation of Hunan Province [14JJ2004, 2013FJ4015]
- Fund from Hunan Provincial Science and Technology Department [2014SK3066]
- Teacher Research Fund of Central South University [2015JSJJ021]
- Fundamental Research Funds for the Central Universities of Central South University [2016zzts483, 2017zzts030]
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The human embryonic stem cell (hESC) line NERCe003-A-1 was generated by introducing lentiviral-vector-mediated tetracycline-inducible beta-catenin expression into a normal hESC line, NERCe003-A. The resulting cell line can overexpress the beta-catenin protein, encoded by the CTNNB1 gene, after exposure to doxycycline (Dox). CTNNB1 gene expression was confirmed by quantitative PCR (qPCR) and immunofluorescence assays. Further characterization confirmed that the NERCe003-A-1 cell line expresses typical pluripotency markers and has the ability to form the three germ layers both in vitro and in vivo. (c) 2018 The Authors. Published by Elsevier B.V.
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