Journal
STEM CELL RESEARCH
Volume 28, Issue -, Pages 11-15Publisher
ELSEVIER
DOI: 10.1016/j.scr.2018.01.017
Keywords
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Funding
- National Basic Research Program of China (973 program) [2012CB944901]
- National Natural Science Foundation of China [81101510]
- Natural Science Foundation of Hunan Province [14JJ2004, 2013FJ4015]
- Fund from Hunan Provincial Science and Technology Department [2014SK3066]
- Teacher Research Fund of Central South University [2015JSJJ021]
- Fundamental Research Funds for the Central Universities of Central South University [2016zzts483, 2017zzts030]
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NERCe002-A-3 cells were generated from the normal human embryonic stem cell line NERCe002-A. NERCe002-A-3 cells overexpressed 14-3-3 zeta after exposure to doxycycline. 14-3-3 zeta protein have the ability to bind a multitude of functionally diverse signalling proteins. The NERCe002-A-3 cell line is considered a model for functional studies of the 14-3-3 zeta protein in hESC self-renewal and cell differentiation. Doxycycline-treated NERCe002-A-3 cells showed a >27-fold increase in relative expression of 14-3-3 zeta as compared with un-induced cells. Characterization assays proved that NERCe002-A-3 cells express typical markers of pluripotency and have the ability to form the three germ layers in vivo. (c) 2018 The Authors. Published by Elsevier B.V.
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