Journal
METABOLITES
Volume 2, Issue 4, Pages 717-732Publisher
MDPI
DOI: 10.3390/metabo2040717
Keywords
metabolomics; metabolite profiles; stringent response; relaxed phenotype
Categories
Funding
- Portuguese FCT (Fundacao para a Ciencia e Tecnologia) [MIT-Pt/BS-BB/0082/2008]
- ERDF-European Regional Development Fund through the COMPETE Programme (operational programme for competitiveness)
- National Funds through the FCT (Portuguese Foundation for Science and Technology) [FCOMP-01-0124-FEDER-009707]
- FCT [SFRH/BD/22863/2005]
- Fundação para a Ciência e a Tecnologia [MIT-Pt/BS-BB/0082/2008, SFRH/BD/22863/2005] Funding Source: FCT
Ask authors/readers for more resources
Metabolite profiling of E. coli W3110 and the isogenic. relA mutant cells was used to characterize the RelA-dependent stringent control of metabolism under different growth conditions. Metabolic profiles were obtained by gas chromatography-mass spectrometry (GC-MS) analysis and revealed significant differences between E. coli strains grown at different conditions. Major differences between the two strains were assessed in the levels of amino acids and fatty acids and their precursor metabolites, especially when growing at the lower dilution rates, demonstrating differences in their metabolic behavior. Despite the fatty acid biosynthesis being the most affected due to the lack of the RelA activity, other metabolic pathways involving succinate, lactate and threonine were also affected. Overall, metabolite profiles indicate that under nutrient-limiting conditions the RelA-dependent stringent response may be elicited and promotes key changes in the E. coli metabolism.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available