Label-free fluorescent aptasensor berberine-based strategy for ultrasensitive detection of Hg2+ ion

A label-free fluorescent aptasensing platform was fabricated and a simple and rapid method to detect Hg2+ ion in aqueous solution was put forward by means of berberine and Hg2+ ion-aptamer are as the fluorescence probe and the recognition element, respectively. Various factors including the concentration of berberine, Hg2+ ion and Hg2+ ion-aptamer, pH effect and the reaction time were investigated in detail. Under the optimal experimental conditions, in the sensing system, the fluorescence intensity changes displayed a calibration response for Hg2+ ion in the range of 0.1 mu M to 10.0 mu M and the detection limit was of 7.7 nM (S/N = 3). The fabricated label-free fluorescence aptasensor is not only conveniently but also effectively applicable used for analysis of Hg2+ ion in blood serum and tap water samples and the recovery range is of 96.0%-105.7%. In brief, this study offers an easy, economical and stable assay system for detecting Hg2+ ion in rough condition. (C) 2018 Elsevier B.V. All rights reserved.