Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 188, Issue -, Pages 197-201Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2017.06.065
Keywords
Fluorescent probe; Nitroreductase; Aggregation-induced emission; Tetraphenylethene; Hypoxia
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Funding
- NSFC [21472067, 21672083]
- Taishan Scholar Foundation [TS 201511041]
- University of Jinan [309-10004]
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The biological activity of nitroreductase (NTR) is closely related to biological hypoxia status in organisms. The development of effective methods for monitoring the activity of NTR is of great significance for medical diagnosis and tumor research. Toward this goal, we have developed a new aggregation-induced emission (AIE) fluorescence NTR probe TPE-HY used the tetraphenylethene as the fluorophore, and used the nitro group as the NTR recognition site. The probe TPE-HY has many excellent properties, including rapid response, AIE characteristics, high sensitivity and selectivity, and low cytotoxicity. Importantly, the probe TPE-HY is successfully applied to monitor endogenous NTR in living HeLa cells. (C) 2017 Elsevier B.V. All rights reserved.
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