Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 203, Issue -, Pages 127-131Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2018.05.121
Keywords
Water-soluble; Mitochondrial targeting; Viscosity sensing; Benzothiazole salt; Zebrafish
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Funding
- Doctoral Startup Foundation of Wannan Medical College [wYRCQD201715]
- Natural Science Foundation of Anhui Province [1508085SMB208]
- Science Foundation of Department Education of Anhui Province [KJ2017ZD49]
- Talent Foundation of the Education Department of Anhui [gxbjZD2016111]
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Rational design of water-soluble probes for mitochondrial viscosity in practical biological applications remains a challenge. Herein, we described a novel hydro soluble benzothiazole salt derivative MitoSN, which exhibits specifically response and singular sensitivity to the mitochondria viscosity in living Hela cells. MitoSN displays an excellent fluorescence enhancement (ca. 35-fold) with the increase of the viscosity in the water-glycerol system. Moreover, confocal microscopy indicates that MitoSN is sensitive to the local viscosity and selectively stains mitochondria, the body of zebrafish as well. Importantly, MitoSN is capable to identify the viscosity difference of mitochondria in normal and nystatin treated Hela cells. The work provides a useful tool to monitor the changes of viscosity in the mitochondrial microenvironment. (C) 2018 Elsevier B.V. All rights reserved.
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