Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 202, Issue -, Pages 346-351Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2018.05.047
Keywords
Triangular silver nanoparticles; Dengue virus; RT-PCR; Serotypes
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There is always a substantial effort to develop a point of care detection fora severe and acute disease like dengue. In this work, we have described the detection of serotype-specific dengue virus using multicolor triangular silver nanopartides (TAg) which could be a potential diagnosis method to distinguish between serotype. Functionalized TAg was prepared with polyA(10) DNA through pH-mediated process. Further, it was characterized with Transmission Electron Microscopy (TEM), Ultra Visible-visible spectroscopy (UV-vis) and gel electrophoresis and its stability towards NaCl concentration. The colorimetric detection was carried out based on the interaction of TAg-DNA probe with specific complimentary strand which was designed to form a network assembly between DNA probes and sample RNA. The hybridization process was enhanced in presence of NaCI (0.4 M) which makes the hybridization process more stable over probe compared to un-hybridization. Therefore a simple and effective colorimetric method was developed to detect the different dengue serotype RNA with high sensitivity and specificity. (C) 2018 Elsevier B.V. All rights reserved.
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