Journal
SOUTH AFRICAN JOURNAL OF BOTANY
Volume 120, Issue -, Pages 261-267Publisher
ELSEVIER
DOI: 10.1016/j.sajb.2018.07.003
Keywords
Extraction; Medicinal plants; Sudan; Total phenolic content; Total flavonoid content; DPPH; alpha-Glucosidase; Pancreatic lipase; Tyrosinase
Categories
Funding
- JSPS RONPAKU (Dissertation PhD) Program
- Program for Leading Graduate Schools, Health life science: Interdisciplinary and Glocal Oriented (HIGO) Program, MEXT, Japan
- Program for Building Regional Innovation Ecosystems at Kumamoto University, Japan
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Solvents used during extraction process are reported to have an influence on the nature and the amount of secondary metabolites extracted from medicinal plants. Thus, the choice of proper extraction solvent is necessary for the desired pharmacological activity of these extracts. In the present study, we extracted six medicinal plants grown in Sudan, namely, Blepharis linariifolia, Cyperus rotundus, Guiera senegalensis, Maerua pseudopetalosa, Tinospora bakis and Dicoma tomentosa with six solvents of different polarity, i.e. water, 50% ethanol (EtOH), 70% EtOH, 95% EtOH, acetone and dichloromethane to obtain total 36 extracts. Quantitative estimation of total phenolic and flavonoid contents was performed. These extracts were further evaluated for their free radical scavenging activities and alpha-glucosidase, pancreatic lipase and tyrosinase inhibitory activities. 50% EtOH and 70% EtOH extracts of G. senegalensis showed the highest content of phenolic compounds in comparison to other extracts. For total flavonoid contents, higher content was found in acetone extracts of G. senegalensis followed by B. linariifolia and M. pseudopetalosa. Polar extracts showed higher free radical scavenging activity in comparison to dichloromethane extract, with 70% EtOH and 50% EtOH extracts of G. senegalensis being the most active ones. The 50% EtOH and 70% EtOH extracts G. senegalensis also showed the potent inhibitory activity against alpha-glucosidase. In contrast, acetone and dichloromethane extracts showed potent pancreatic lipase inhibitory activity. Potent tyrosinase inhibitory activity was shown by acetone extract of G. senegalensis. In conclusion, 50% EtOH, 70% EtOH and acetone were found to be the best solvents of choice for the extraction of phenolic compound rich extracts with free radical scavenging and enzyme inhibitory activities. Further studies should focus on the isolation and identification of active secondary metabolites from these extracts. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.
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